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KMID : 0379119980260010103
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Korean Journal of Mycology
1998 Volume.26 No. 1 p.103 ~ p.107
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The Studies on the Heterogenous Gene Expression in Schizophyllum commune
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Abstract
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The trp2 gene encoding trifunctional enzyme in Coprinus cinereus was investigated the expression in the heterothallic mushroom species. To identify the homology of trp2 gene to Schizophyllum commune and Pleurotus ostreatus, southern hybridization was performed with plasmid pHIONA8 containing C. cinereus trp2 gene as a probe, which resulted in a strong signal indicating an homologous sequence to the chromosomal DNA of S. commune. About 50 transformants per dish was appeared in the complementation test by pHIONA8 using S. commune tryptophan auxotroph as host. In the mating test between transformants and other mating type alleles, the fruiting body of S. commune was formed at 30℃ in 2∼3 weeks.
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